Review





Similar Products

centrin2  (Proteintech)
93
Proteintech centrin2
Centrin2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/centrin2/product/Proteintech
Average 93 stars, based on 1 article reviews
centrin2 - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
pcw mpgk smim27 hrv3c 2xmyc hpgk mirfp670 centrin2  (Addgene inc)
96
Addgene inc pcw mpgk smim27 hrv3c 2xmyc hpgk mirfp670 centrin2
Pcw Mpgk Smim27 Hrv3c 2xmyc Hpgk Mirfp670 Centrin2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcw mpgk smim27 hrv3c 2xmyc hpgk mirfp670 centrin2/product/Addgene inc
Average 96 stars, based on 1 article reviews
pcw mpgk smim27 hrv3c 2xmyc hpgk mirfp670 centrin2 - by Bioz Stars, 2026-04
96/100 stars
  Buy from Supplier
rabbit monoclonal centrin2 cheeseman lab83  (Proteintech)
93
Proteintech rabbit monoclonal centrin2 cheeseman lab83
Rabbit Monoclonal Centrin2 Cheeseman Lab83, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit monoclonal centrin2 cheeseman lab83/product/Proteintech
Average 93 stars, based on 1 article reviews
rabbit monoclonal centrin2 cheeseman lab83 - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
pegfp centrin2  (Addgene inc)
93
Addgene inc pegfp centrin2
Pegfp Centrin2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pegfp centrin2/product/Addgene inc
Average 93 stars, based on 1 article reviews
pegfp centrin2 - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
mouse antibodies against centrin2 04-1624  (Merck KGaA)
90
Merck KGaA mouse antibodies against centrin2 04-1624
Generation of CEP215-truncated mutants with defects in molecular dynamicity. (A) Schematic diagram illustrating the CEP215 protein with functional sites (CM1 and CM2), protein-binding sites, coiled-coil domains (CCDs), intrinsically disordered regions (IDRs) and the truncated mutant sites used in this study. (B) The number of cells with (w/) cytoplasmic clusters of FLAG–CEP215–CRY2-truncated mutant proteins was counted. * P <0.05 (one-way ANOVA with Tukey's post hoc test). (C) FRAP signals of the mCh–CEP215–CRY2 mutant proteins at the cytoplasm were measured for up to 600 s. w/o, without. (D) Stably expressed FLAG–mCh–CEP215 mutants in p53 KO ; CEP215 KO HeLa cells were subjected to immunoblot analyses with anti-CEP215, FLAG and GAPDH antibodies. Blot representative of four experimental repeats. (E) FRAP analyses of the FLAG–mCh–CEP215 proteins were carried out at mitotic phase centrosomes of the rescued cells. <t>GFP–centrin2</t> marks the centrosomes. The analyzed cells were photographed with differential interference contrast microscopy. Scale bars: 1 μm (FRAP); 10 μm (DIC). (F) The FRAP signals of FLAG–mCh–CEP215 (WT, ΔCCD5 and L1867S) at mitotic phase centrosomes were measured for up to 160 s. For B, C and F, at least 90 (B), 30 (C) and 10 (F) cells per experimental group were counted in three independent experiments. Values are mean±s.e.m. C and F show arbitrary units.
Mouse Antibodies Against Centrin2 04 1624, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse antibodies against centrin2 04-1624/product/Merck KGaA
Average 90 stars, based on 1 article reviews
mouse antibodies against centrin2 04-1624 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
centrin2-gfp cb6-tg(cag-egfp/cetn2)3-4jgg/j  (Jackson Laboratory)
90
Jackson Laboratory centrin2-gfp cb6-tg(cag-egfp/cetn2)3-4jgg/j
Generation of CEP215-truncated mutants with defects in molecular dynamicity. (A) Schematic diagram illustrating the CEP215 protein with functional sites (CM1 and CM2), protein-binding sites, coiled-coil domains (CCDs), intrinsically disordered regions (IDRs) and the truncated mutant sites used in this study. (B) The number of cells with (w/) cytoplasmic clusters of FLAG–CEP215–CRY2-truncated mutant proteins was counted. * P <0.05 (one-way ANOVA with Tukey's post hoc test). (C) FRAP signals of the mCh–CEP215–CRY2 mutant proteins at the cytoplasm were measured for up to 600 s. w/o, without. (D) Stably expressed FLAG–mCh–CEP215 mutants in p53 KO ; CEP215 KO HeLa cells were subjected to immunoblot analyses with anti-CEP215, FLAG and GAPDH antibodies. Blot representative of four experimental repeats. (E) FRAP analyses of the FLAG–mCh–CEP215 proteins were carried out at mitotic phase centrosomes of the rescued cells. <t>GFP–centrin2</t> marks the centrosomes. The analyzed cells were photographed with differential interference contrast microscopy. Scale bars: 1 μm (FRAP); 10 μm (DIC). (F) The FRAP signals of FLAG–mCh–CEP215 (WT, ΔCCD5 and L1867S) at mitotic phase centrosomes were measured for up to 160 s. For B, C and F, at least 90 (B), 30 (C) and 10 (F) cells per experimental group were counted in three independent experiments. Values are mean±s.e.m. C and F show arbitrary units.
Centrin2 Gfp Cb6 Tg(Cag Egfp/Cetn2)3 4jgg/J, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/centrin2-gfp cb6-tg(cag-egfp/cetn2)3-4jgg/j/product/Jackson Laboratory
Average 90 stars, based on 1 article reviews
centrin2-gfp cb6-tg(cag-egfp/cetn2)3-4jgg/j - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
mcherry centrin2 n 10  (Addgene inc)
93
Addgene inc mcherry centrin2 n 10
Generation of CEP215-truncated mutants with defects in molecular dynamicity. (A) Schematic diagram illustrating the CEP215 protein with functional sites (CM1 and CM2), protein-binding sites, coiled-coil domains (CCDs), intrinsically disordered regions (IDRs) and the truncated mutant sites used in this study. (B) The number of cells with (w/) cytoplasmic clusters of FLAG–CEP215–CRY2-truncated mutant proteins was counted. * P <0.05 (one-way ANOVA with Tukey's post hoc test). (C) FRAP signals of the mCh–CEP215–CRY2 mutant proteins at the cytoplasm were measured for up to 600 s. w/o, without. (D) Stably expressed FLAG–mCh–CEP215 mutants in p53 KO ; CEP215 KO HeLa cells were subjected to immunoblot analyses with anti-CEP215, FLAG and GAPDH antibodies. Blot representative of four experimental repeats. (E) FRAP analyses of the FLAG–mCh–CEP215 proteins were carried out at mitotic phase centrosomes of the rescued cells. <t>GFP–centrin2</t> marks the centrosomes. The analyzed cells were photographed with differential interference contrast microscopy. Scale bars: 1 μm (FRAP); 10 μm (DIC). (F) The FRAP signals of FLAG–mCh–CEP215 (WT, ΔCCD5 and L1867S) at mitotic phase centrosomes were measured for up to 160 s. For B, C and F, at least 90 (B), 30 (C) and 10 (F) cells per experimental group were counted in three independent experiments. Values are mean±s.e.m. C and F show arbitrary units.
Mcherry Centrin2 N 10, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mcherry centrin2 n 10/product/Addgene inc
Average 93 stars, based on 1 article reviews
mcherry centrin2 n 10 - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier

Image Search Results


Generation of CEP215-truncated mutants with defects in molecular dynamicity. (A) Schematic diagram illustrating the CEP215 protein with functional sites (CM1 and CM2), protein-binding sites, coiled-coil domains (CCDs), intrinsically disordered regions (IDRs) and the truncated mutant sites used in this study. (B) The number of cells with (w/) cytoplasmic clusters of FLAG–CEP215–CRY2-truncated mutant proteins was counted. * P <0.05 (one-way ANOVA with Tukey's post hoc test). (C) FRAP signals of the mCh–CEP215–CRY2 mutant proteins at the cytoplasm were measured for up to 600 s. w/o, without. (D) Stably expressed FLAG–mCh–CEP215 mutants in p53 KO ; CEP215 KO HeLa cells were subjected to immunoblot analyses with anti-CEP215, FLAG and GAPDH antibodies. Blot representative of four experimental repeats. (E) FRAP analyses of the FLAG–mCh–CEP215 proteins were carried out at mitotic phase centrosomes of the rescued cells. GFP–centrin2 marks the centrosomes. The analyzed cells were photographed with differential interference contrast microscopy. Scale bars: 1 μm (FRAP); 10 μm (DIC). (F) The FRAP signals of FLAG–mCh–CEP215 (WT, ΔCCD5 and L1867S) at mitotic phase centrosomes were measured for up to 160 s. For B, C and F, at least 90 (B), 30 (C) and 10 (F) cells per experimental group were counted in three independent experiments. Values are mean±s.e.m. C and F show arbitrary units.

Journal: Journal of Cell Science

Article Title: Enhancement of CEP215 dynamics for spindle pole assembly during mitosis

doi: 10.1242/jcs.263542

Figure Lengend Snippet: Generation of CEP215-truncated mutants with defects in molecular dynamicity. (A) Schematic diagram illustrating the CEP215 protein with functional sites (CM1 and CM2), protein-binding sites, coiled-coil domains (CCDs), intrinsically disordered regions (IDRs) and the truncated mutant sites used in this study. (B) The number of cells with (w/) cytoplasmic clusters of FLAG–CEP215–CRY2-truncated mutant proteins was counted. * P <0.05 (one-way ANOVA with Tukey's post hoc test). (C) FRAP signals of the mCh–CEP215–CRY2 mutant proteins at the cytoplasm were measured for up to 600 s. w/o, without. (D) Stably expressed FLAG–mCh–CEP215 mutants in p53 KO ; CEP215 KO HeLa cells were subjected to immunoblot analyses with anti-CEP215, FLAG and GAPDH antibodies. Blot representative of four experimental repeats. (E) FRAP analyses of the FLAG–mCh–CEP215 proteins were carried out at mitotic phase centrosomes of the rescued cells. GFP–centrin2 marks the centrosomes. The analyzed cells were photographed with differential interference contrast microscopy. Scale bars: 1 μm (FRAP); 10 μm (DIC). (F) The FRAP signals of FLAG–mCh–CEP215 (WT, ΔCCD5 and L1867S) at mitotic phase centrosomes were measured for up to 160 s. For B, C and F, at least 90 (B), 30 (C) and 10 (F) cells per experimental group were counted in three independent experiments. Values are mean±s.e.m. C and F show arbitrary units.

Article Snippet: For immunostaining analyses, we used rabbit antibodies against CEP215/CDK5RAP2 (06-1398; Merck Millipore; 1:100), PCNT , CEP192 (A302-324A; Bethyl Laboratory; 1:100), α-tubulin (ab18251; Abcam), γ-tubulin (ab11317; Abcam; 1:100); mouse antibodies against centrin2 (04-1624; Merck Millipore), α-tubulin (T6199; Sigma; 1:100), FLAG (F3165; Sigma; 1:100); and goat anti-FLAG (ab1257; Abcam; 1:100) antibodies.

Techniques: Functional Assay, Protein Binding, Mutagenesis, Stable Transfection, Western Blot, Microscopy